Embryo Freezing

Freezing is done by a method called Vitrification,it is the rapid cooling of liquid medium in the absence of ice crystal formation. The solution forms an amorphous glass as a result of rapid cooling by direct immersion of the embryos in a polyethelene (PE) straw into liquid nitrogen. The glass retains the normal molecular/ionic distributions of a liquid but remains in an extremely viscous, supercooled form. Vitrification is a simple and less expensive alternative to conventional freezing.

Steps of Vitrification and thawing are:

In vitrification procedures, cell dehydration is affected by direct exposure to concentrated vitrifying solutions prior to cooling in liquid nitrogen.

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