Freezing is done by a method called Vitrification, it is the rapid cooling of liquid medium in the absence of ice crystal formation.
The solution forms an amorphous glass as a result of rapid cooling by direct immersion of the embryos in a polyethelene (PE) straw into liquid nitrogen.
The glass retains the normal molecular/ionic distributions of a liquid but remains in an extremely viscous, supercooled form.
Vitrification is a simple and less expensive alternative to conventional freezing.
Any patient can take advantage of laser-assisted hatching, but those most likely to be best suited are those who:
» Are above the age of 37
» Produce a high level of Follicle
» Stimulating Hormone (FSH) early in their cycle
» Have had unsuccessful IVF cycles
» Have a tendency to produce a harder and/or thicker zona pellucida
Steps of Vitrification and thawing are:
Steps of Vitrification and thawing are:
In vitrification procedures, cell dehydration is affected by direct exposure to concentrated vitrifying solutions prior to cooling in liquid nitrogen.
Egg freezing is a medical procedure that involves harvesting and preserving a woman’s eggs (oocytes) for potential future use. The process typically involves stimulating the ovaries to produce multiple
eggs, retrieving them, and then freezing and storing the eggs for an extended period.
Social egg freezing, also known as elective oocyte cryopreservation, has emerged as a revolutionary option for women to preserve their fertility for non-medical reasons. This process allows them to postpone childbearing while retaining the potential to conceive using their eggs in the future. In recent years, there has been a notable increase in oocyte cryopreservation worldwide, driven by advancements in vitrification techniques and regulatory approvals from organizations like the HFEA
(Human Fertilisation and Embryology Authority) and ASRM (The American Society for Reproductive Medicine).
A recent study conducted at Guy’s Hospital sheds light on the outcomes of egg thaw cycles following social egg freezing. It revealed that the mean age at egg freezing was 37.1 years. Despite the growing number of egg-freezing cycles, only 16% of women returned to utilize their frozen eggs. However, the study did find a promising live birth rate per embryo transfer, comparable to fresh IVF cycles.
Additionally, a literature review encompassing ten global studies emphasized the significance of age at freezing and the number of eggs frozen. Younger age and a higher number of frozen eggs were associated with better outcomes. On average, the worldwide live birth rate following oocyte cryopreservation stood at 35% per embryo transfer.
While social egg freezing offers significant opportunities for women, concerns regarding usage rates and cost-effectiveness persist. Personalized counselling, informed by data on outcomes and success
factors, is essential to assist women in making well-informed decisions about this reproductive option.
Egg freezing involves six essential steps that enable women to preserve their fertility for future use.
Hormonal treatment prompts ovaries to produce multiple eggs
Hormone levels and follicle development are closely observed
Mature eggs are retrieved through a surgical procedure
Eggs are rapidly cooled to prevent damage
Frozen eggs are stored in a cryogenic facility
Eggs are thawed, fertilized, and transferred for implantation
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